LOCUS       LARGE2-pGEn2            7383 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_recomb     complement(3839..3867)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    6011..6552
                     /note="WPRE"
     misc_recomb     5973..5995
                     /label=attB2
                     /note="attB2 site"
                     /note="recognized as attB2"
     misc_feature    complement(6559..6773)
                     /note="BGH pA"
     misc_feature    complement(6861..6990)
                     /note="3'-ITR"
     misc_feature    2982..3056
                     /note="TCM signal sequence"
     misc_feature    3060..3083
                     /note="His tag"
     misc_feature    3084..3134
                     /note="AviTag"
     misc_feature    3135..3830
                     /note="Superfolder GFP"
     misc_feature    2976..2981
                     /note="Kozak seq"
     misc_feature    3868..3890
                     /note="TEV site"
     misc_feature    3891..5966
                     /note="OPTIMIZED LARGE2 coding region"
     vector          join(5981..7383,1..3857)
                     /source="pGEn2-DEST"
                     /type="Destination Vector"
     clone           3858..5980
                     /source="LARGE2 pDONR"
                     /type="Entry Clone"
     source          1..7383
                     /dnas_title="LARGE2-pGEn2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC
     3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC
     3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA
     3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG
     3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG
     3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC
     3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG
     3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC
     3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG
     3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA
     3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA
     3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG
     3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
     3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG
     3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT
     3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc GACCTGGGCT
     3901 GCGAGAGAAG AGAACCAGGT GGTAGAGCTG GCGCCCCTGG ATGTTTTCCT GGACCTCTGA
     3961 TGCCCAGAGT GCCCCCTGAT GGTAGACTGA GAAGGGCTGC TGCTCTGGAT GGCGATCCTG
     4021 GTGCTGGACC TGGCGACCAC AATAGAAGCG ATTGTGGACC TCAGCCCCCT CCACCTCCTA
     4081 AGTGTGAACT GCTGCACGTG GCCATTGTGT GTGCCGGCCA CAATTCCAGC AGAGATGTGA
     4141 TCACCCTGGT CAAGAGCATG CTGTTCTACC GGAAGAACCC CCTGCATCTG CACCTGGTCA
     4201 CAGACGCCGT GGCCAGAAAT ATCCTGGAAA CCCTGTTCCA CACCTGGATG GTGCCTGCTG
     4261 TGCGGGTGTC CTTTTATCAC GCCGATCAGC TGAAGCCCCA GGTGTCCTGG ATCCCCAACA
     4321 AGCACTATAG CGGCCTGTAC GGCCTGATGA AGCTGGTCCT GCCTTCTGCT CTGCCTGCCG
     4381 AACTGGCTAG AGTGATCGTG CTGGATACCG ACGTGACCTT CGCCAGCGAT ATCTCTGAGC
     4441 TGTGGGCCCT GTTCGCCCAC TTTAGCGATA CACAGGCCAT CGGCCTGGTG GAAAACCAGA
     4501 GCGACTGGTA TCTGGGCAAC CTGTGGAAGA ATCACAGACC TTGGCCTGCC CTGGGAAGAG
     4561 GCTTTAACAC AGGCGTGATC CTGCTGCGGC TGGATAGACT CAGACAGGCC GGATGGGAAC
     4621 AGATGTGGCG GCTGACAGCT AGAAGAGAGC TGCTGAGCCT GCCTGCTACA AGCCTGGCCG
     4681 ACCAGGACAT CTTCAACGCC GTGATCAAAG AGCACCCTGG CCTGGTCCAG AGACTGCCTT
     4741 GTGTCTGGAA TGTGCAGCTG TCCGATCACA CACTGGCCGA GAGATGTTAC AGCGAGGCCA
     4801 GCGACCTGAA AGTGATCCAC TGGAACAGCC CCAAGAAACT GAGAGTGAAG AACAAACACG
     4861 TGGAATTCTT CAGAAACTTC TACCTGACCT TCCTGGAATA CGACGGCAAT CTGCTGAGGC
     4921 GCGAGCTGTT TGTGTGTCCT TCACAGCCTC CTCCAGGTGC TGAGCAGCTC CAGCAGGCCC
     4981 TGGCTCAGCT GGATGAGGAA GATCCCTGCT TCGAGTTCAG ACAGCAGCAG CTGACAGTGC
     5041 ACAGAGTGCA CGTGACATTT CTGCCTCACG AGCCTCCACC CCCAAGACCT CATGATGTGA
     5101 CACTGGTGGC CCAGCTGTCT ATGGACAGAC TCCAGATGCT GGAAGCACTG TGTAGACATT
     5161 GGCCAGGCCC TATGTCTCTG GCCCTGTATC TGACAGATGC CGAGGCCCAG CAGTTTCTGC
     5221 ACTTTGTGGA AGCTAGCCCT GTGCTGGCCG CTAGACAGGA TGTGGCCTAT CACGTGGTGT
     5281 ACAGAGAGGG ACCCCTGTAC CCTGTGAACC AGCTGCGAAA TGTGGCTCTG GCACAGGCAC
     5341 TGACCCCTTA CGTGTTCCTG AGCGACATCG ACTTTCTGCC CGCCTACTCC CTGTACGACT
     5401 ACCTGAGAGC CTCTATCGAG CAGCTGGGAC TGGGCTCTAG AAGAAAAGCT GCACTCGTGG
     5461 TGCCAGCCTT CGAGACACTG CGGTACAGAT TCAGCTTCCC CCACAGCAAG GTGGAACTGC
     5521 TCGCTCTCCT GGATGCCGGC ACCCTGTATA CCTTCAGATA TCACGAGTGG CCTAGAGGCC
     5581 ACGCCCCTAC AGATTATGCT AGATGGCGAG AAGCCCAGGC CCCCTATAGA GTGCAGTGGG
     5641 CCGCCAATTA CGAGCCCTAC GTGGTGGTGC CTAGGGACTG CCCTAGATAC GATCCCAGAT
     5701 TCGTGGGCTT CGGCTGGAAC AAAGTGGCCC ACATTGTCGA ACTGGACGCC CAGGAATATG
     5761 AACTCCTGGT CCTCCCTGAG GCCTTTACCA TTCATCTGCC ACACGCCCCC AGCCTGGACA
     5821 TCTCTAGATT CAGAAGCTCC CCCACCTACC GGGACTGTCT GCAGGCACTC AAGGACGAGT
     5881 TTCACCAGGA CCTGAGCAGA CACCATGGCG CCGCTGCACT GAAATATCTG CCAGCTCTGC
     5941 AGCAGCCTCA GAGCCCAGCT AGAGGATAGG ACCCAGCTTT CTTGTACAAA GTGGTTGATA
     6001 GCTTGGATCC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA
     6061 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT
     6121 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA
     6181 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC
     6241 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT
     6301 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG
     6361 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC
     6421 ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC
     6481 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT
     6541 TCCGCGTCTT CGAGATCTGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC
     6601 CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA
     6661 AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG
     6721 GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGACTCGAGT
     6781 TAAGGGCGAA TTGCCGATTA GGATCTTCCT AGAGCATGGC TACGTAGATA AGTAGCATGG
     6841 CGGGTTAATC ATTAACTACA AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG
     6901 CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG
     6961 GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CCTTAATTAA GAGCTCATGG CGCGCCTAGG
     7021 CCTTGACGGC CTTCCGCCAA TTCGCCCTAT AGTGAGTCGT ATTACGTCGC GCTCACTGGC
     7081 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC
     7141 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG AAACGCCCTT
     7201 CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGGAGCGCCC TGTAGCGGCC ACTCAACCCT
     7261 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA
     7321 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT
     7381 TAG
//

